Abstract: Objective To study the effect of Naoshuantong capsule on angiogenesis in rats with focal cerebral ischemia reperfusion. Method Healthy adult male SD rats were induced to develop occlusion of the middle cerebral artery by suture embolus, Then, the rats were randomly divided into the sham group, a model group, and a low-dose Naoshuantong capsule and high-dose Naoshuantong capsule groups ( 1. 05, 2. 62 g / kg, respectively) . Naoshuantong capsule was administered once per day for 7 days, from 24 h after surgery a day. To examine the recovery of neurological function, we measured modified neurological severity score (mNss) method ; the infarction volume was measured by 2, 3, 5-triphenyltetrazolium chloride ( TTC) staining technique; Western blot was used to detect the expression of angiogenesis related proteins in rat cerebral cortex; Angiogenesis was detected by immunofluorescence staining. Result Compared with those in the sham group,the scores of neurological behavior and the cerebral infarction volume ratios in the model group were significantly increased ( P < 0. 001) . Compared with that in the model group,the low and high-dose Naoshuantong capsule groups exhibited a dramatic improvement of the neurological behavior scores ( P < 0. 05 ) , Naoshuantong capsule high-dose groups exhibited a significant reduction in the volume of cerebral infarction ( P < 0. 05 ) . Compared with the model group, the expression of cortical angiogenesis-related proteins vascular endothelial growth factor (VEGF) and basic fibroblast growth factor ( bFGF) in the Naoshuantong capsule low and high-dose groups were significantly increased( VEGF:P< 0. 05,P< 0. 001;bFGF:P < 0. 05,P < 0. 001) ; Compared with the model group, the number of Lectin + / Ki67 + cellls were significantly increased ( P < 0. 05, P < 0. 001 ) . Conclusion Naoshuantong capsule may promote angiogenesis to improve neurological function after ischemia in rats.

Key words: Naoshuantong capsule, cerebral ischemia-reperfusion, neurological function, cerebral infarction volume, angiogenesis

摘要： 目的 研究脑栓通胶囊对局灶性脑缺血再灌注模型大鼠血管新生的影响。 方法 用线栓法制备局灶性脑缺血再灌注大鼠模型,术后随机分为假手术组、模型组和脑栓通胶囊小(1. 05 g / kg) 、大(2. 62 g / kg)剂量给药组,术后24 h 开始给予脑栓通胶囊,每天灌胃 1 次,连续给药 7 d。 采用 mNss 法评估各组大鼠的神经功能缺损情况,TTC 染色检测脑梗死体积百分比,Western blot 检测大鼠大脑皮层血管新生相关蛋白的表达,免疫荧光染色检测新生血管。结果 术后 7 d,与假手术组相比,模型组 mNss 评分和脑梗死体积显著增加(均 P<0. 001) ;与模型组相比,小剂量给药组和大剂量给药组神经功能损伤有显著改善( P<0. 05) ,大剂量给药组脑梗死体积显著减少( P<0. 05) 。 与模型组相比,小剂量给药组和大剂量给药组血管内皮生长因子( vascular endothelial growth factor, VEGF) 和碱性成纤维细胞生长因子 ( basic fibroblast growth factor, bFGF) 表达水平均显著增加 ( VEGF:P < 0. 05,P < 0. 001; bFGF:P <0. 05,P<0. 001) ;与模型 组 相 比,小剂量给药组和大剂量给药组血管新生数量显著增加( P < 0. 05, P < 0. 001) 。结论 脑栓通胶囊可以通过促进血管新生改善大鼠缺血后神经功能。

关键词: 脑栓通胶囊, 脑缺血再灌注, 神经功能, 脑梗死体积, 血管新生